Using a set of post-vaccination sera, trainees will carry out virus neutralisation test (VNT) and a solid-phase competition ELISA (SPCE). By the end of the week, results for both the VNT and the ELISA will be compared with the goal of determining the serostatus of the animals from which the samples were collected. The course will provide guidance on how to establish these tests in trainee’s laboratory and will also outline the quality assurance steps needed to decrease variation and monitor the performance of these assays (with the support from the Pirbright Quality Assurance team). The use of these tests to support post vaccination monitoring studies and PD50/PPG vaccine potency studies will be discussed with the Pirbright team.
What you need to know
- 1-week course
- £1,250 ex VAT
- Limited to 4 participants
- The Plowright Building
Who Should Attend
Laboratory staff or managers using or wanting to use serological assays to assess the immune response of livestock after vaccination with foot-and-mouth disease (FMD) vaccine.
Requirements and Qualifications
The course is hands-on with trainees receiving practical training in virus neutralisation test (VNT) and ELISA methods; therefore, the trainee must be competent in basic laboratory techniques such as pipetting. Previous experience working in a high containment laboratory is recommended although not a requirement.
In order for delegates to benefit as much as possible from this training course, and to satisfy Health and Safety rules in our high containment facilities (BSL3), all applicants are required to be able to demonstrate their ability to communicate in English. Where English is not the first language, a formal qualification is required. The standard is set as an overall IELTS score of 6.5 with no less than 6.0 in listening, reading and speaking and no less than 5.5 in writing. Alternative qualifications at a similar level are acceptable, e.g. TOEFL, CEF. Please email email@example.com before applying if you require further information.
- Set-up and demonstrate the VNT and SPCE - including when these assays should be used and what their advantages and limitations are.
- Be able to implement the quality assurance processes needed to decrease variation and monitor the performance of these assays.
- Understand the use of these tests to assess the performance of FMD vaccines (when samples are collected in post-vaccination monitoring studies and PD50/PPG experiments).