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Research paper

Ultrastructural development of Theileria annulata in the salivary gland of Hyalomma anatolicum anatolicum

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Research paper

Organ culture of Rhipicephalus appendiculatus with maturation of Theileria parva in tick salivary glands in vitro

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Research paper

Artificial infection of the tick Rhipicephalus appendiculatus with Theileria parva

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Research paper

Rapid quantitative assessment of Theileria infection in ticks

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Research paper

A high-frequency polymorphism in exon 6 of the CD45 tyrosine phosphatase gene (PTPRC) resulting in altered isoform expression

CD45 (leukocyte common) antigen is a hemopoietic cell-specific tyrosine phosphatase essential for antigen receptor-mediated signaling in lymphocytes. The molecule undergoes complex alternative splicing in the extracellular domain, and different patterns of CD45 splicing are…
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Research paper

Development of a polyclonal competitive enzyme-linked immunosorbent assay for detection of antibodies to Ehrlichia ruminantium

A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E. ruminantium as the antigen and biotin-labeled polyclonal goat…
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Research paper

Mouse respiratory epithelial cells support efficient replication of human rhinovirus

Human rhinoviruses (HRV) are responsible for the majority of virus infections of the upper respiratory tract. Furthermore, HRV infection is associated with acute exacerbation of asthma and other chronic respiratory diseases of the lower respiratory tract. A small animal…
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Research paper

Early platelet aggregation as a cause of thrombocytopenia in classical swine fever

Twenty pigs were inoculated with a virulent classical swine fever virus isolate: to determine the mechanism responsible for thrombocytopenia using histopathologic, ultrastructural, and immunohistochemical (detection of viral antigens gp55 and FVIII-rag) techniques. In…
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Research paper

Transcriptional analysis of the major antigenic protein I multigene family of Cowdria ruminantium

The major antigenic protein I (MAPI) of the tick-borne rickettsial pathogen Cowdria ruminantium is encoded by a multigene family containing conserved and variable genes. The part of a focus containing the map1 multigene family that was characterized contained three…
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Research paper

Immunogenicity of Ehrlichia ruminantium grown in tick cell lines

Ehrlichia (previously Cowdria) ruminantium, the pathogen which causes heartwater in domestic and wild ruminants, can now be propagated in cell lines from one vector (Amblyomma variegatum) and five non-vector (Ixodes scapularis, I. ricinus, Boophilus decoloratus, B. microplus…
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