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An enzyme-linked immunosorbent assay (ELISA) using sporozoite, schizont and piroplasm antigens was developed to study the immune response of animals that had been immunised with either Theileria annulata sporozoites or schizont-infected cells and then challenged with…

This work extends basic knowledge of tropical theileriosis in taurine and crossbred cattle. Infection of Bos taurus and Bos taurus cross BON indicus (Sahiwal) calves with graded doses of sporozoites of Theileria annulata (Hissar), an Indian stock of the parasite, showed the…

The establishment of 5 continuous cell lines from embryonic tissues of H. a. anatolicum is reported. Each line comprises 2 or more cell types; they are maintained at 28 and 32°C in L-15/H-Lac medium with 20% fetal calf serum, and have been cryopreserved successfully…

The development at 28 degrees C of Theileria annulata (Hissar) in the salivary glands of its tick vector, Hyalomma anatolicum anatolicum, was studied using Giemsa-stained smears, methyl green-pyronin-stained preparations of whole glands, and electron microscopy. Nymphs which…