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Real-time PCR for differential quantification of CVI988 vaccine virus and virulent strains of Mareks disease virus

CVI988/Rispens vaccine, the gold standard vaccine against Mareks disease in poultry, is not easily distinguishable from virulent strains of Mareks disease herpesvirus (MDV). Accurate differential measurement of CVI988 and virulent MDV is commercially important to confirm…

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Using shared needles for subcutaneous inoculation can transmit bluetongue virus mechanically between ruminant hosts

Bluetongue virus (BTV) is an economically important arbovirus of ruminants that is transmitted by Culicoides spp. biting midges. BTV infection of ruminants results in a high viraemia, suggesting that repeated sharing of needles between animals could result in its iatrogenic…

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Experimental infection of Ornithodoros erraticus sensu stricto with two Portuguese African swine fever virus strains. Study of factors involved in the dynamics of infection in ticks

African swine fever (ASF) is a frequently devastating hemorrhagic disease of domestic pigs and wild boar and Ornithodoros erraticus sensu stricto argasid ticks are the only biological vectors of African swine fever virus (ASFV) known to occur in Europe. Recently this disease…

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Efficient generation of vesicular stomatitis virus (VSV)-pseudotypes bearing morbilliviral glycoproteins and their use in quantifying virus neutralising antibodies

Morbillivirus neutralising antibodies are traditionally measured using either plaque reduction neutralisation tests (PRNTs) or live virus microneutralisation tests (micro-NTs). While both test formats provide a reliable assessment of the strength and specificity of the…

Pirbright scientists inspire at Innovate

On Saturday 12 March 2016, scientists from Pirbright headed to Innovate Guildford – a free festival of science and arts, where they showcased their research on influenza and insects. The Innovate Guildford science and arts festival is part of the Mayor of Guildford…

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Mass vaccination, immunity and coverage: modelling population protection against foot-and-mouth disease in Turkish cattle

Foot-and-mouth disease (FMD) in Turkey is controlled using biannual mass vaccination of cattle. However, vaccine protection is undermined by population turnover and declining immunity. A dynamic model of the Turkish cattle population was created. Assuming biannual mass…

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The non-structural protein 5 and matrix protein are antigenic targets of T cell immunity to genotype 1 porcine reproductive and respiratory syndrome viruses

The porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of one of the most economically important diseases affecting swine worldwide. Efforts to develop a next-generation vaccine have largely focused on envelope glycoproteins to target virus-neutralizing…

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Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses

We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a…

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Vaccines against peste des petits ruminants virus

Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of sheep, goats and wild ruminants. The disease is controlled by the use of live-attenuated vaccines that give a lifelong immunity. Currently, several PPR vaccines are available for use in the…

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Immunochemical characterization of in vitro culture-derived antigens of Babesia bovis and Babesia bigemina

Cross-reactivity between Babesia bovis and B. bigemina becomes a problem in discrimination of the two infections in endemic areas where the two species usually occur in association. With the aim of identifying candidate proteins for use as specific diagnostic tools, culture…