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Drosophila 5′ → 3′-exoribonuclease Pacman

This chapter concentrates on the methods used to express a Drosophila recombinat 5? ? 3?-exoribonuclease, purify the protein, and analyze its activity in vitro. Analysis of early development in Drosophila has shown that RNA localization, control of translation, and mRNA…

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Use of plum pox potyvirus as an expression vector in plants

Plum pox potyvirus (PPV) is the causal agent of a devastating disease that affects stone fruit trees of the Prunus genus. However, it is also able to infect herbaceous hosts from several different families. This broad host range confers PPV an added value regarding its…

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Morphological and immunohistochemical changes in splenic macrophages of pigs infected with classical swine fever

Classical swine fever (CSF) was induced in 20 pigs by inoculation with a virulent strain of CSF virus to determine sequential changes (2, 4, 7, 10 and 14 days post-inoculation) in the number and morphology of splenic macrophages (red pulp and lymphoid marginal zone) and thus…

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Proinflammatory cytokine expression by Theileria annulata infected cell lines correlates with the pathology they cause in vivo

Control of Theileria annulata is currently best achieved by the use of live attenuated cell line vaccines. However, the mechanisms underlying attenuation are unclear and there is a need to rapidly produce new cell line vaccines, which could safely and effectively vaccinate…

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Down-regulated lymphoproliferation coincides with parasite maturation and with the collapse of both gamma interferon and interleukin-4 responses in a bovine model of onchocerciasis

Onchocerciasis is a debilitating parasitic infection caused by the filarial nematode Onchocerca volvulus. Infections are chronic, and persistence of the parasites for several years argues for highly adapted mechanisms of immune evasion. Due to the restricted host repertoire…

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The coronavirus infectious bronchitis virus nucleoprotein localizes to the nucleolus

The coronavirus nucleoprotein (N) has been reported to be involved in various aspects of virus replication. We examined by confocal microscopy the subcellular localization of the avian infectious bronchitis virus N protein both in the absence and in the context of an…

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Detection of monokines in paraffin-embedded tissues of pigs using polyclonal antibodies

Monokines are glycoproteins, synthesised by macrophages, which exert various effects on the organism. The most important monokines are interleukin (IL)-1 alpha, IL-1 beta, tumor necrosis factor (TNF)-alpha and IL-6. This paper reports on immunohistochemical techniques…

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The Drosophila gene twister, an orthologue of the yeast helicase SKI2, is differentially expressed during development

We have identified and characterized a Drosophila orthologue of SK12, which, in Saccharomyces cerevisiae. is one of the key components in the cytoplasmic 3 ' -5 ' decay of mRNA. The Drosophila orthologue (twister, tst), is expressed as two transcripts which differ in the…

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A point mutation in CD45 may be associated with an increased risk of HIV-1 infection

The CD45 antigen is essential for normal antigen receptor-mediated signalling in lymphocytes, and different patterns of splicing of CD45 are associated with distinct functions in lymphocytes. Here we show that abnormal CD45 splicing caused by a C77G transversion in exon A of…

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The Exon A (C77G) mutation is a common cause of abnormal CD45 splicing in humans

The leukocyte common (CD45) Ag is essential for normal T lymphocyte function and alternative splicing at the N terminus of the gene is associated with changes in T cell maturation and differentiation. Recently, a statistically significant association was reported in a large…