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Antiviral effect of lithium chloride on replication of avian leukosis virus subgroup J in cell culture

Lithium chloride (LiCl) has been reported to possess antiviral activity against several viruses. In the current study, we assessed the antiviral activity effect of LiCl on ALV-J infection in CEF cells by using real-time PCR, Western blot analysis, IFA and p27 ELISA analysis…

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Implementation of tissue histopathology and parasitic morphometric analysis in the diagnosis of Myxobolus fomenai infection in the skeletal muscles Nile tilapia

Class Myxosporea includes Myxobolus parasite. Myxosporean species could infect the skeletal muscles of freshwater fish, including Nile tilapia. In this study, four Nile tilapia fish, collected from a fish farm in Dakahleya province, died and showed gross lesions of…

Pirbright gains funding to tackle lumpy skin disease

The Pirbright Institute’s Dr Pip Beard has been awarded funding to study lumpy skin disease (LSD), a severe viral disease of cattle which is found throughout Africa and the Middle East. Little is known about the LSD virus, so the award will be used to answer fundamental…

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Detection of pathogen exposure in African buffalo using non-specific markers of inflammation

Detecting exposure to new or emerging pathogens is a critical challenge to protecting human, domestic animal and wildlife health. Yet current techniques to detect infections typically target known pathogens of humans or economically important animals. In the face of the…

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The third moments of the site frequency spectrum

The analysis of patterns of segregating (i.e. polymorphic) sites in aligned sequences is routine in population genetics. Quantities of interest include the total number of segregating sites and the number of sites with mutations of different frequencies, the so-called site…

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Internal genes of a highly pathogenic H5N1 influenza virus determine high viral replication in myeloid cells and severe outcome of infection in mice

The highly pathogenic avian influenza (HPAI) H5N1 influenza virus has been a public health concern for more than a decade because of its frequent zoonoses and the high case fatality rate associated with human infections. Severe disease following H5N1 influenza infection is…

Marek's disease diagnostic testing

Diagnostic testing for Marek's disease.

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African swine fever: A re-emerging viral disease threatening the global pig industry

African swine fever (ASF) recently has spread beyond sub-Saharan Africa to the Trans-Caucasus region, parts of the Russian Federation and Eastern Europe. In this new epidemiological scenario, the disease has similarities, but also important differences, compared to the…

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A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system

Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines…

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Association of Marek's disease induced immunosuppression with activation of a novel regulatory T cell

Marek's Disease Virus (MDV) is an alphaherpesvirus that infects chickens, transforms CD4+ T cells and causes deadly lymphomas. In addition, MDV induces immunosuppression early during infection by inducing cell death of the infected lymphocytes, and potentially due to…