A recent study carried out at The Pirbright Institute has uncovered the sites within the cell where porcine deltacoronavirus (PDCoV) makes new copies of itself during replication. Using electron microscopy, Pirbright scientists demonstrated that the virus creates replication organelles that are similar to other coronaviruses. Establishing that this step in the virus life cycle is consistent across the virus family could facilitate the development of broad-spectrum control methods to tackle the diseases they cause.
Researchers at The Pirbright Institute have used the CRISPR/Cas9 gene editing tool to knock out a gene in Culex quinquefasciatus Say, an understudied species of mosquito that spreads a variety of bird, animal and human diseases, including West Nile virus. The study provides the first characterisation of a gene that determines eye colour in this species, which will prove a valuable marker for researchers looking to modify this mosquito to control wild populations and aid disease prevention.
Researchers from The Pirbright Institute have shown that glutamine, a protein building block, is essential for the replication of Marek’s disease virus in chickens. Their study revealed that cells infected with the virus were unable to survive glutamine deprivation, a finding that could be used to develop treatments for the disease.