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Identification of protein interacting partners using tandem affinity purification

A critical and often limiting step in understanding the function of host and viral proteins is the identification of interacting cellular or viral protein partners. There are many approaches that allow the identification of interacting partners, including the yeast two…

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High-resolution functional profiling of the norovirus genome

Human noroviruses (HuNoV) are a major cause of nonbacterial gastroenteritis worldwide, yet details of the life cycle and replication of HuNoV are relatively unknown due to the lack of an efficient cell culture system. Studies with murine norovirus (MNV), which can be…

Genetically altered African swine fever virus shows promise as a vaccine

Scientists at The Pirbright Institute have found that deleting a gene of the African swine fever virus (ASFV), a severe and often fatal disease of pigs, reduces its ability to cause infection and protects against a strain of the virus that causes severe disease. In the study…

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Genetic economy in picornaviruses: foot-and-mouth disease virus replication exploits alternative precursor cleavage pathways

The RNA genomes of picornaviruses are translated into single polyproteins which are subsequently cleaved that into structural and non-structural protein products. For genetic economy, proteins and processing intermediates have evolved to perform distinct functions. The…

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Structures of foot and mouth disease virus pentamers: Insight into capsid dissociation and unexpected pentamer reassociation

This is the first structure of dissociated pentamers for FMDV, and indeed for any picornavirus. We also present the first visualization of a picornavirus particle assembled from pentamers comprising VP1, 2 and 3. The formation of regular assemblies rather than random…

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Deletion of the African swine fever virus gene DP148R does not reduce virus replication in culture but reduces virus virulence in pigs and induces high levels of protection against challenge

Many of the approximately 165 proteins encoded by African swine fever virus do not have significant similarity to known proteins and have not been studied experimentally. One such protein is DP148R. We showed that the DP148R gene is transcribed at early times post-infection…

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Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136154)] linked to a T-cell epitope [3A(2135)] of FMDV confers…

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Pirbright retains Athena SWAN Bronze award

The Pirbright Institute has retained the Bronze award from the Equality Challenge Unit’s (ECU) Athena SWAN Charter for its commitment to gender equality. The Institute has undergone significant changes in leadership and structure since it first attained a Bronze award in…

Dr Bryan Charleston, Director, The Pirbright Institute

Director's vision

Safeguarding UK livestock, global health and food security.

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How often do mosquitoes bite humans in southern England? A standardised summer trial at four sites reveals spatial, temporal and site-related variation in biting rates

This field-based study examined the abundance and species complement of mosquitoes (Diptera: Culicidae) attracted to humans at four sites in the United Kingdom (UK). The study used a systematic approach to directly measure feeding by mosquitoes on humans at multiple sites…