Dengue virus

Dengue virus (DENV) is a member of the Flavivirus genus and Flaviviridae family of viruses. DENV is an arthropod-borne virus (arbovirus) and is primarily transmitted by mosquitoes. Like other flaviviruses, DENV contains a genome composed of single stranded positive-sense RNA, which is surrounded by a capsid and a lipid envelope derived from the host cell. There are four dengue viruses (DENV-1, DENV-2, DENV-3 and DENV-4), and the body’s antibody-mediated immune response can cross-react between these four viruses (or “serotypes”) to cause increased disease severity.

There is no specific antiviral therapy for dengue, and the currently available treatments aim to reduce the severity of symptoms. A vaccine is available in some countries, but is not recommended for use in all scenarios.

Associated diseases

The World Health Organisation classifies dengue disease into three clinical categories: dengue without warning signs (dengue fever), dengue with warning signs, and severe dengue (sometimes referred to as dengue haemorrhagic fever and/or dengue shock syndrome). 

Clinical signs:

The majority of DENV infections are asymptomatic or result in a flu-like febrile illness (“dengue fever”) that usually subsides with no further complications. Symptoms typically begin 5-8 days after the initial point of infection. However, in rare cases, severe dengue, which is serious and potentially fatal condition, can develop.

Dengue fever symptoms include:

  • Fever
  • Severe headache
  • Pain behind the eyes
  • Muscle and joint pain
  • Nausea
  • Diarrhoea
  • Rashes (typically on the abdomen)
  • Stomach ache/loss of appetite

Typical warning signs that a patient may develop severe dengue include:

  • Abdominal pain or tenderness
  • Persistent vomiting
  • Rapid breathing
  • Bleeding from gums and other mucosal surfaces
  • Fatigue
  • Restlessness
  • Clinical fluid accumulation
  • Liver enlargement

Severe dengue is potentially fatal and typically involves:

  • Vascular leakage
  • Haemorrhage
  • Circulatory shock

Disease transmission:

DENV is primarily spread by infected mosquitoes, specifically the tropical species Aedes aegypti and Aedes albopictus. DENV can also spread from pregnant women to the foetus via the placenta. Furthermore, DENV has also been reported to spread through blood transfusion and organ transplantation.

Disease prevalence:

DENV is found across all tropical and subtropical regions of the world where its mosquito vectors are present, including Latin America and the Caribbean, Asia, Africa, and Oceania.  Local transmission in parts of North America and Europe has also been reported, and the virus is expected to expand its range as its mosquito vectors move into new areas due to climate change.

Impact for Society – what are we doing?

We are carrying out basic science research into how DENV establishes infection in the mosquito vector and what barriers exist that influence the mosquito-borne transmission of DENV. DENV needs to be able to infect and replicate in both mosquito and human cells; we study the virus in both systems to understand the constraints and requirements for replication in these two different environments. We are also using our fundamental insights into vector-virus interactions to try to create new barriers to transmission, for example virus-resistant mosquitoes. In the longer term this could help us develop new ways of preventing human infection by blocking viral transmission.

Research papers

Hamlin R E, Rahman A, Pak T R, Maringer K, Mena I, Bernal-Rubio D, Potla U, Maestre A M, Fredericks A C, Amir E D, Kasarskis A, Ramos I, Merad M, Fernandez-Sesma A (2017)

JCI Insight 2 (13) , e92424
Aguirre S, Maestre A M, Pagni S, Patel J R, Savage T, Gutman D, Maringer K, Bernal-Rubio D, Shabman R S, Simon V, Rodriguez-Madoz J R, Mulder L C, Barber G N, Fernandez-Sesma A (2012)

PLoS Pathogens 8 (10) , e1002934
Taguwa S, Maringer K, Li X, Bernal-Rubio D, Rauch J N, Gestwicki J E, Andino R, Fernandez-Sesma A, Frydman J (2015)

Cell 163 (5) , 1108-1123
Fredericks A C, Russell T A, Wallace L E, Davidson A D, Fernandez-Sesma A, Maringer K (2019)

PLoS Neglected Tropical Diseases 13 (11) , e0007346
Cheung Y P, Mastrullo V, Maselli D, Butsabong T, Madeddu P, Maringer K, Campagnolo P (2020)

mSphere 5 (4) , e00258-20

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