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Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source

The increasing prevalence of insecticide resistance and the ongoing global burden of vector-borne diseases have encouraged new efforts in mosquito control. For Aedes aegypti, the most important arboviral vector, integration rates achieved in Cas9-based knock-ins so far have…

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p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling

Many cellular genes and networks induced in human lung epithelial cells infected with the influenza virus remain uncharacterized. Here, we find that p21 levels are elevated in response to influenza A virus (IAV) infection, which is independent of p53. Silencing…

Avian influenza virus

Avian influenza virus (AIV): eLearning

Learn about avian influenza, how it spreads and how to diagnose.

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Pirbright scientist Kevin Maringer to chair the Microbiology Society’s Members Panel promoting diversity and inclusion in science

The Microbiology Society has formed a new Members Panel to promote equality and diversity in the microbiology community. Pirbright’s Dr Kevin Maringer, group leader of the Flavivirus Transmission and Pathogenesis group, will co-chair the Members Panel which will ensure that…

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Birnaviridae virus factories show features of liquid-liquid phase separation, and are distinct from paracrystalline arrays of virions observed by electron microscopy

To gain more information about the nature of Birnaviridae virus factories (VFs), we used a recombinant infectious bursal disease virus (IBDV) expressing split-GFP11 tagged to the polymerase (VP1) that we have previously shown is a marker for VFs in infected cells expressing…

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Reverse-transcriptase loop-mediated isothermal amplification has high accuracy for detecting SARS-CoV-2 in saliva and naso/oropharyngeal swabs from asymptomatic and symptomatic individuals

Previous studies have described reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) methodology for the rapid detection of SARS-CoV-2 in nasopharyngeal (NP) and oropharyngeal (OP) swab and saliva samples. This study describes the validation of an improved…

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Targeted deletion of glycoprotein B gene by CRISPR/Cas9 nuclease inhibits Gallid herpesvirus type 3 in dually-infected Marek's disease virus-transformed lymphoblastoid cell line MSB-1

Marek's disease virus (MDV) is a member of the genus Mardivirus in the subfamily Alphaherpesvirinae. There are 3 different serotypes of MDV designated as MDV-1 ( Gallid herpesvirus type 2), MDV-2 ( Gallid herpesvirus type 3), and MDV-3 ( Meleagrid herpesvirus 1, herpesvirus…

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Targeted deletion of glycoprotein B gene by CRISPR/Cas9 nuclease inhibits Gallid herpesvirus type 3 in dually-infected Marek's disease virus-transformed lymphoblastoid cell line MSB-1

Marek's disease virus (MDV) is a member of the genus Mardivirus in the subfamily Alphaherpesvirinae. There are 3 different serotypes of MDV designated as MDV-1 ( Gallid herpesvirus type 2), MDV-2 ( Gallid herpesvirus type 3), and MDV-3 ( Meleagrid herpesvirus 1, herpesvirus…

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The Aedes aegypti Domino ortholog p400 regulates antiviral exogenous small interfering RNA pathway activity and ago-2 expression

Arboviruses are pathogens of humans and animals. A better understanding of the interactions between these pathogens and the arthropod vectors, such as mosquitoes, that transmit them is necessary to develop novel control measures. A major antiviral pathway in the mosquito…

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The natural cytotoxicity receptor NKp44 (NCR2, CD336) is expressed on the majority of porcine NK cells ex vivo without stimulation

Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural…