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Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva

An infection and treatment protocol is used to vaccinate cattle against Theileria parva infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of…

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MAP4K3 modulates cell death via the post-transcriptional regulation of BH3-only proteins

Intracellular signal transduction networks involving protein kinases are important modulators of cell survival and cell death in multicellular organisms. Functional compromise of these networks has been linked to aberrant apoptosis in diseases such as cancer. To identify…

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Mechanism of inactivation of NF-κB by a viral homologue of IκBα: signal-induced release of IκBα results in binding of the viral homologue to NF-κB

Activation of the nuclear factor κB plays a key role in viral pathogenesis, resulting in inflammation and modulation of the immune response. We have previously shown that A238L, an open reading frame from African swine fever virus (ASFV), encoding a protein with 40% homology…

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Potency of a thermostabilised chimpanzee adenovirus Rift Valley Fever vaccine in cattle

Development of safe and efficacious vaccines whose potency is unaffected by long-term storage at ambient temperature would obviate major vaccine deployment hurdles and limit wastage associated with breaks in the vaccine cold chain. Here, we evaluated the immunogenicity of a…

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Protection of cattle against rinderpest by vaccination with wild type but not attenuated strains of peste des petits ruminants virus

Although Rinderpest virus (RPV) has been eradicated in the wild, efforts are still continuing to restrict the extent to which live virus is distributed in facilities around the world, and to prepare for any reappearance of the disease, whether through deliberate or…

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Reverse genetics of avian coronavirus infectious bronchitis virus

We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a…

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Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India

Bluetongue virus (BTV) infects all ruminants, including cattle, goats and camelids, causing bluetongue disease (BT) that is often severe in naïve deer and sheep. Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assays were developed to detect eastern or…

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Sensitivity of African swine fever virus to type I interferon is linked to genes within multigene families 360 and 505

African swine fever virus (ASFV) causes a lethal haemorrhagic disease of pigs. There are conflicting reports on the role of interferon in ASFV infection. We therefore analysed the interaction of ASFV with porcine interferon, in vivo and in vitro. Virulent ASFV induced…

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The characterisation of bovine plasmacytoid dendritic cells and their interaction with foot and mouth disease virus (FMDV) and bovine viral diarrhoea virus (BVDV) - (PhD Thesis: University of Surrey)

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The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens

Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to…