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Research paper

Strategies for sequencing of genome of bluetongue virus isolates

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Research paper

Genome sequence of a reassortant strain of bluetongue virus serotype 23 from Western India

The full genome sequence (19,177 bp) of an Indian strain (IND1988/02) of bluetongue virus (BTV) serotype 23 was determined. This virus was isolated from a sheep that had been killed during a severe bluetongue outbreak that occurred in Rahuri, Maharashtra State, western India…
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Research paper

MicroRNA expression profiles in avian haemopoietic cells

MicroRNAs (miRNAs) are small, abundant, non-coding RNAs that modulate gene expression by interfering with translation or stability of mRNA transcripts in a sequence-specific manner. A total of 734 precursor and 996 mature miRNAs have so far been identified in the chicken…
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Research paper

Avian paramyxoviruses serotype 1 to 10

This chapter discusses the up-to-date literature on historical distribution, virus and disease, classification, viral replication, pathogenesis, clinical signs, gross lesions, disease transmission, other avian paramyxovirus serotypes and laboratory diagnosis of Avian…
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Research paper

Sequential deletion of genes from the African swine fever virus genome using the cre/loxP recombination system

A method has been established to sequentially delete combinations of genes from the ASFV genome to test the effect on virus replication and host responses to infection. Initially the ASFV genes MGF505 2R and MGF505 3R and a truncated MGF360 9L gene were deleted from the…
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Research paper

Emergence of foot-and-mouth disease virus SAT 2 in Egypt during 2012

The epidemiology of foot-and-mouth disease (FMD) in North Africa is complicated by the co-circulation of endemic FMD viruses (FMDV), as well as sporadic incursions of exotic viral strains from the Middle East and Sub-Saharan Africa. This report describes the molecular…
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Research paper

Detection and identification of putative bacterial endosymbionts and endogenous viruses in tick cell lines

As well as being vectors of many viral, bacterial, and protozoan pathogens of medical and veterinary importance, ticks harbour a variety of microorganisms which are not known to be pathogenic for vertebrate hosts. Continuous cell lines established from ixodid and argasid…
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Research paper

Tick cell culture isolation and growth of Rickettsia raoultii from Dutch Dermacentor reticulatus ticks

Tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. In an attempt to derive continuous Dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks…
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Research paper

Characterisation of full-length cDNA sequences provides insights into the Eimeria tenella transcriptome

Background: Eimeria tenella is an apicomplexan parasite that causes coccidiosis in the domestic fowl. Infection with this parasite is diagnosed frequently in intensively reared poultry and its control is usually accorded a high priority, especially in chickens raised for…
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Research paper

Control of the olive fruit fly using genetics-enhanced sterile insect technique

Background: The olive fruit fly, Bactrocera oleae, is the major arthropod pest of commercial olive production, causing extensive damage to olive crops worldwide. Current control techniques rely on spraying of chemical insecticides. The sterile insect technique (SIT) presents…
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