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Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus

Coronavirus sub-genomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (TRSs), one (TRS-L) encompassing the leader sequence of the 5' untranslated region (UTR), and the other upstream of…

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Evaluation of cell lines for the isolation of foot-and-mouth disease virus and other viruses causing vesicular disease

The most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in…

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A novel antiviral formulation inhibits a range of enveloped viruses

Some free fatty acids derived from milk and vegetable oils are known to have potent antiviral and antibacterial properties. However, therapeutic applications of short- to medium-chain fatty acids are limited by physical characteristics such as immiscibility in aqueous…

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The Veterinary Immunological Toolbox: Past, Present and Future

It is well-recognized that research capability in veterinary species is restricted by a lack of immunological reagents relative to the extensive toolboxes for small rodent biomedical model species and humans. This creates a barrier to the strategic development of disease…

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Immune escape adaptive mutations in the H7N9 avian influenza hemagglutinin protein increase virus replication fitness and decrease pandemic potential

H7N9 avian influenza viruses (AIVs) continue to evolve and remain a huge threat to human health and the poultry industry. Previously, serially passaging the H7N9 A/Anhui/1/2013 virus in the presence of homologous ferret antiserum resulted in immune escape viruses containing…

avian flu virus

Pirbright shows bird flu mutations could increase threat to poultry, but reduce potential to infect humans

Mutations in the H7N9 bird flu virus increase its replication rate and stability in avian cells, but reduce its preference for infecting human cells, a study from The Pirbright Institute has found. The results show that outbreaks in birds caused by strains with these…

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Marek's disease virus in Egypt: historical overview and current research based on the major mdv-encoded oncogene meq

An update survey of the MDV strains circulating in the Egyptian poultry flocks during 2015 -2016 was conducted. Twenty one vaccinated chicken flocks suffering from different form of lymphomas, representing different governorates in the Egyptian Delta, were screened for MDV…

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Evaluation of commercial qPCR kits for detection of SARS-CoV-2 in pooled samples

Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their…

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Temperature sensitivity; a potential method for the generation of vaccines against the avian coronavirus infectious bronchitis virus

The Gammacoronavirus infectious bronchitis virus (IBV) is a highly contagious economically important respiratory pathogen of domestic fowl. Reverse genetics allows for the molecular study of pathogenic determinants to enable rational vaccine design. The recombinant IBV (rIBV…

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Temperature sensitivity; a potential method for the generation of vaccines against the avian coronavirus infectious bronchitis virus

The Gammacoronavirus infectious bronchitis virus (IBV) is a highly contagious economically important respiratory pathogen of domestic fowl. Reverse genetics allows for the molecular study of pathogenic determinants to enable rational vaccine design. The recombinant IBV (rIBV…