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Hepatocyte growth factor (HGF) binding domains on fibronectin and vitronectin promote enhanced endothelial cell migration via a specific ras and PI3-kinase dependent pathway

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Detection of FMD infection in vaccinated animals

The purpose of this paper is to briefly review the state of development of tests for the detection of FMD infection in vaccinated animals and the guidelines that are available for their validation and ultimate use. A brief update will be given on studies to develop and…

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Insertion of a foreign sequence on capsid surface loops of human papillomavirus type 16 virus-like particles reduces their capacity to induce neutralizing antibodies and delineates a conformational neutralizing epitope

The aims of this study were to generate chimeric human papillomavirus (HPV)-16 L1 virus-like particles (VLPs) in order to identify immunogenic domains and conformational neutralizing epitopes, and to characterize the regions where a foreign epitope could be introduced. We…

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A histopathologic, immunohistochemical, and ultrastructural study of the intestine in pigs inoculated with classical swine fever virus

The aim of this study was to report on the lesions occurring in the intestine during experimental classical swine fever (CSF) and to clarify the nature of infected cells and the distribution of viral antigen. Thirty-two pigs were inoculated with the virulent CSF virus (CSFV)…

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A high-frequency polymorphism in exon 6 of the CD45 tyrosine phosphatase gene (PTPRC) resulting in altered isoform expression

CD45 (leukocyte common) antigen is a hemopoietic cell-specific tyrosine phosphatase essential for antigen receptor-mediated signaling in lymphocytes. The molecule undergoes complex alternative splicing in the extracellular domain, and different patterns of CD45 splicing are…

Biological Safety Cabinet - copyright The Pirbright Institute

Biological safety cabinets (Class I and Class II) for users: eLearning

Learn how Class I and Class II biological safety cabinets (BSCs) work and good operator practices.

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Development of a polyclonal competitive enzyme-linked immunosorbent assay for detection of antibodies to Ehrlichia ruminantium

A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E. ruminantium as the antigen and biotin-labeled polyclonal goat…

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Mouse respiratory epithelial cells support efficient replication of human rhinovirus

Human rhinoviruses (HRV) are responsible for the majority of virus infections of the upper respiratory tract. Furthermore, HRV infection is associated with acute exacerbation of asthma and other chronic respiratory diseases of the lower respiratory tract. A small animal…

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Early platelet aggregation as a cause of thrombocytopenia in classical swine fever

Twenty pigs were inoculated with a virulent classical swine fever virus isolate: to determine the mechanism responsible for thrombocytopenia using histopathologic, ultrastructural, and immunohistochemical (detection of viral antigens gp55 and FVIII-rag) techniques. In…

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Transcriptional analysis of the major antigenic protein I multigene family of Cowdria ruminantium

The major antigenic protein I (MAPI) of the tick-borne rickettsial pathogen Cowdria ruminantium is encoded by a multigene family containing conserved and variable genes. The part of a focus containing the map1 multigene family that was characterized contained three…