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In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates

Ehrlichia canis, the etiologic agent of canine ehrlichiosis, is an obligate intracytoplasmic Gram-negative tick-borne bacterium belonging to the Anaplasmataceae family. E. canis is distributed worldwide and can cause serious and fatal infections in dogs. Among strains of E…

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Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus

African swine fever virus (ASFV) causes an acute haemorrhagic disease of domestic pigs against which there is no effective vaccine. The attenuated ASFV strain OUR T88/3 has been shown previously to protect vaccinated pigs against challenge with some virulent strains…

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Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013)

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Recently agreed changes to the International Code of Virus Classification and Nomenclature

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Genetic control of Aedes mosquitoes

Aedes mosquitoes include important vector species such as Aedes aegypti, the major vector of dengue. Genetic control methods are being developed for several of these species, stimulated by an urgent need owing to the poor effectiveness of current methods combined with an…

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Serosurveillance of orbiviruses in wild cervids from Spain (Letter)

In recent years, several emerging vectorborne diseases, including bluetongue (BTV-1, BTV-4 and BTV-8), Bagaza, Schmallenberg, Usutu and West Nile, have been reported in southern Spain, most of them originating from north Africa. Although EHDV circulation has been recently…

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Coronavirus reverse genetics

This chapter contains sections titled: The Coronavirinae, Infectious bronchitis, Coronavirus genome organisation, The coronavirus replication cycle, Development of reverse genetics system for coronaviruses including IBV, Reverse genetics system for IBV, Reverse genetics…

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Relationship between levels of very virulent MDV in poultry dust and in feather tips from vaccinated chickens

To assess the effect of various vaccine strains on replication and shedding of virulent Marek's disease virus from experimentally infected chickens, quantitative PCR (q-PCR) methods were developed to accurately quantify viral DNA in infected chickens and in the environment…

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Dugbe virus ovarian tumour domain interferes with ubiquitin/ISG15-regulated innate immune cell signalling

The ovarian tumour (OTU) domain of the nairovirus L protein has been shown to remove ubiquitin and interferon-stimulated gene 15 protein (ISG15) from host cell proteins, which is expected to have multiple effects on cell signalling pathways. We have confirmed that the OTU…

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Creation of a completely helper cell-dependent recombinant morbillivirus

We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P?) grew…