Diagnosis of peste des petits ruminants

This is a laboratory based course that is designed for laboratory technicians or those who have limited knowledge of peste des petits ruminants virus (PPRV) and who will be responsible for implementing diagnostic techniques within the laboratory.  This course is not suitable for research or group leaders.  The course focuses on diagnostic techniques including serological, molecular and virological methods of detection. Participants should have an understanding of virology and related methodologies.

What you need to know

Cost: £1000

Duration: 1 week, dates TBC

Accommodation: The Pirbright Institute has access to basic accommodation in shared houses at an additional cost of approximately £30 per night (excluding food). The number of rooms is however limited and availability is on a first come – first served basis and cannot therefore be guaranteed. The alternative is for course delegates to use local Bed & Breakfast accommodation which we can help them to find.

Biosecurity: The laboratory operates at BSL3 and delegates will be expected to adhere to tight biosecurity regulations, including a strict three day quarantine and a shower upon exiting the laboratories daily. Further information wil be provided.

Places are limited.  Applications close TBC.

Course pre requisites

In order for delegates to achieve the most possible from this training course, and to ensure their Health and Safety in the high containment facilities (BSL3) at The Pirbright Institute, all applicants are required to be able to demonstrate their ability in English language.  Where English is not the first language, a formal qualification is required. The standard is set as an overall IELTS score of 6.5 with no less than 6.0 in any sub-section.  Alternative qualifications at a similar level are acceptable, e.g. TOEFL, CEF.  Please email pirbright.etraining@pirbright.ac.uk before applying if you require further information.

Participants requiring a VISA will need a full business visa.

Learning Objectives

The course will provide a thorough understanding of current diagnostic techniques for PPRV, including discusions regarding QA and sample management. In addition, the course will discuss the aetiology, clinical signs and pathology, immunity, epidemiology, control and eradication of the disease. The course will be delivered through a series of seminars, practical demonstrations and hands-on practical work in the BSL3  laboratory.

Course Description

Topics to be discussed include:

Virus isolation – PPRV

  • Passage of PPRV on cell culture  

Diagnostic tests – PPRV

  • PPRV Antibody detection ELISA
  • Serum neutralisation test
  • Manual nucleic acid extraction
  • Real-time RT-PCR  
  • F gene RT-PCR
  • F gene nucleotide sequencing and lineage determination

Course material (CD and / or on-line) containing seminars and standard operating proceedures will be provided at the end of the course.

About the trainers

The PPRV training course will be hosted by the Non-vesicular Disease Reference Laboratories (NVRL), led by Dr Carrie Batten.

The OIE, FAO and national reference laboratories for PPRV form a part of the NVRL at Pirbright. The Pirbright Institute was heavily involved in the global eradication of Rinderpest virus (RPV) and has used this experience to build a strong research program into PPRV. The OIE expert for PPRV, Dr Michael Baron, and other reasearch leaders will present their most current findings and discuss the implications for disease control.

How to apply

Further information will be available once the course dates have been published.

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