This is a laboratory based course that is designed for laboratory staff or those who have a limited knowledge of foot-and-mouth disease virus (FMDV) and who will be responsible for implementing FMDV diagnostic techniques within the laboratory. This course is not suitable for research or group leaders. The course focuses on FMDV diagnostic techniques including serological, molecular and virological methods of detection. Participants should have an understanding of virology and related methodologies.
Course pre requisites
Participants requiring a visa to enter the UK will need a full business visa.
Demonstrations and practicals on:
- Sample receipt and preparation for FMD virus/antigen/genome detection
- Use of cell cultures for FMD virus isolation
- ELISA for FMD virus antigen detection
- Use of a lateral flow device for FMD diagnosis
- RT-PCR for FMD virus genome detection and VP1 sequencing
- FMD virus antibody detection by non-structural protein (NSP) ELISA, liquid phase blocking ELISA (LPBE) and virus neutralisation test
- Background introduction
- Clinical (and differential diagnosis) of FMD
- Epidemiology of FMD
- Ageing of lesions
- Problems with diagnosis in sheep
- Laboratory diagnosis of FMD
- Sample collection, packing and dispatch
- Biosecurity and biosafety in the laboratory
- Quality issues in laboratories dealing with FMD virus
- ELISA practical problems
- Sequencing of FMDV
- FMDV strain characterisation – vaccine matching
Course material (USB stick and/or on-line) containing seminars and standard operating procedures will be provided at the end of the course.
About the trainers
The FMDV training course will be hosted by the World Reference Laboratory for FMD (WRL-FMD).
The WRL-FMD is responsible for the EU, OIE, FAO and national reference laboratory for FMDV and forms part of the Livestock Viral Diseases (LVD) Programme at The Pirbright Institute.
How to apply
Past participants' comments
“Overall I thought the course was fantastic, with really good input from all of the tutors giving great suggestions for the hands on testing.”
“I would like to congratulate you because the course is well organised, and you can tell that the tutors have a lot of experience working with FMDV and moreover all of you have been very friendly.”
“…Pirbright supplies Uganda with FMD diagnostic kits. Through this course we have learnt where there are gaps in our SOPs and we are certain that our kits will now work effectively.”